Review



eclipse ti e c2 confocal laser scanning microscope  (Nikon)


Bioz Verified Symbol Nikon is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Nikon eclipse ti e c2 confocal laser scanning microscope
    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon <t>Eclipse</t> <t>Ti-E</t> <t>C2+</t> <t>microscope.</t> Scale bar = 20 μm.
    Eclipse Ti E C2 Confocal Laser Scanning Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ti+e+microscope/C2%2B/pmc12811640-78-16-23
    Average 99 stars, based on 1 article reviews
    eclipse ti e c2 confocal laser scanning microscope - by Bioz Stars, 2026-09
    99/100 stars

    Images

    1) Product Images from "Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7"

    Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7

    Journal: Biofilm

    doi: 10.1016/j.bioflm.2025.100335

    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope. Scale bar = 20 μm.
    Figure Legend Snippet: Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope. Scale bar = 20 μm.

    Techniques Used: Staining, Mutagenesis, Fluorescence, Microscopy

    Related Articles

    Microscopy:

    Article Title: A Site-Specific Self-Association of a Protein Hub Drives Its Phase Separation.
    Article Snippet: The nuclei were stained with DAPI (Thermo Fischer Scientific, Cat # 62248). .. Images were taken with a spinning-disc confocal instrument (Yokogawa CSU-W1 50 μm 60 Pinhole, Nikon) on an inverted Ti-E microscope (Nikon, Japan) with a 100× oil-immersion objective (1.4 NA). ..

    Article Title: Impact of physiological ionic strength and crowding on kinesin-1 motility
    Article Snippet: Images were acquired with an oil immersion objective lens (Alpha Plan-Apochromat 100×/1.46 OilDIC M27 Elyra, Zeiss) in the Airyscan Fast mode. .. Motility assays were performed using a homebuilt total internal reflection fluorescence microscope based on a Ti-E microscope (Nikon Eclipse) equipped with an oil immersion objective lens (CFI Apochromat TIRF 60 × C Oil, NA/1.49) and a motorized stage (H117E1N4, Prior), as described previously ( Ibusuki et al. , 2022 ). ..

    Article Title: Genetic determinants of pOXA-48 plasmid maintenance and propagation in Escherichia coli.
    Article Snippet: .. At OD600∼0.4–0.5, 0.2% arabinosewas added, and cells were imaged after 240min of induction using a Ti-E microscope (Nikon) equippedwith a 100xphase contrast objective (Plan apo λ 1.45, Nikon). ..

    Article Title: FHOD3 and DIAPH3 control cell migration and differentially shift the balance of parallel and perpendicular stress fibers
    Article Snippet: To improve image resolution, Prolong Gold mounting media (Thermofisher Scientific) was applied between the substrate with cells and the cover slides. .. Samples were sealed with VALAP (a mixture of Vaseline, lanolin, and paraffin) and imaged the next day using epifluorescence microscopy with 10x ( NA =0.3, Nikon, Nishioi, Shinagawa-ku, Japan), 20x ( NA =0.45, Nikon) and 40x ( NA = 1.3, Nikon) objectives on a Ti-E microscope (Nikon) with a CMOS (Moment, Photometrics, Tuscan, AZ). ..

    Article Title: Genetic Determinants of pOXA-48 Plasmid Maintenance and Propagation in Escherichia coli
    Article Snippet: .. At OD 600 ∼0.4-0.5, 0.2% arabinose was added, and cells were imaged after 240 min of induction using a Ti-E microscope (Nikon) equipped with a 100x phase contrast objective (Plan apo λ 1.45, Nikon). ..

    Article Title: A Site-Specific Self-Association of a Protein Hub Drives Its Phase Separation
    Article Snippet: The nuclei were stained with DAPI (Thermo Fischer Scientific, Cat # 62248). .. Images were taken with a spinning-disc confocal instrument (Yokogawa CSU-W1 50 μm 60 Pinhole, Nikon) on an inverted Ti-E microscope (Nikon, Japan) with a 100× oil-immersion objective (1.4 NA). ..

    Article Title: Disruption of constitutive CXCR4 oligomers impairs oncogenic properties in lymphoid neoplasms.
    Article Snippet: .. Imaging was performed on a Ti- E microscope (Nikon) equipped with a 100x Apo TIRF oil immersion objective (NA 1.49) and Perfect Focus System 3 (Nikon). .. Acquired stacks were analyzed using v.1.2.1 of a custom ImageJ plugin called DoM (Detection of Molecules) (https://github.com/ ekatrukha/DoM_Utrecht), as previously described (61).

    Article Title: Genetic determinants of pOXA-48 plasmid maintenance and propagation in Escherichia coli
    Article Snippet: .. At OD 600 ∼0.4–0.5, 0.2% arabinose was added, and cells were imaged after 240 min of induction using a Ti-E microscope (Nikon) equipped with a 100x phase contrast objective (Plan apo λ 1.45, Nikon). ..

    Fluorescence:

    Article Title: Impact of physiological ionic strength and crowding on kinesin-1 motility
    Article Snippet: Images were acquired with an oil immersion objective lens (Alpha Plan-Apochromat 100×/1.46 OilDIC M27 Elyra, Zeiss) in the Airyscan Fast mode. .. Motility assays were performed using a homebuilt total internal reflection fluorescence microscope based on a Ti-E microscope (Nikon Eclipse) equipped with an oil immersion objective lens (CFI Apochromat TIRF 60 × C Oil, NA/1.49) and a motorized stage (H117E1N4, Prior), as described previously ( Ibusuki et al. , 2022 ). ..

    Epifluorescence Microscopy:

    Article Title: FHOD3 and DIAPH3 control cell migration and differentially shift the balance of parallel and perpendicular stress fibers
    Article Snippet: To improve image resolution, Prolong Gold mounting media (Thermofisher Scientific) was applied between the substrate with cells and the cover slides. .. Samples were sealed with VALAP (a mixture of Vaseline, lanolin, and paraffin) and imaged the next day using epifluorescence microscopy with 10x ( NA =0.3, Nikon, Nishioi, Shinagawa-ku, Japan), 20x ( NA =0.45, Nikon) and 40x ( NA = 1.3, Nikon) objectives on a Ti-E microscope (Nikon) with a CMOS (Moment, Photometrics, Tuscan, AZ). ..

    Imaging:

    Article Title: Disruption of constitutive CXCR4 oligomers impairs oncogenic properties in lymphoid neoplasms.
    Article Snippet: .. Imaging was performed on a Ti- E microscope (Nikon) equipped with a 100x Apo TIRF oil immersion objective (NA 1.49) and Perfect Focus System 3 (Nikon). .. Acquired stacks were analyzed using v.1.2.1 of a custom ImageJ plugin called DoM (Detection of Molecules) (https://github.com/ ekatrukha/DoM_Utrecht), as previously described (61).



    Similar Products

    99
    Nikon eclipse ti e c2 confocal laser scanning microscope
    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon <t>Eclipse</t> <t>Ti-E</t> <t>C2+</t> <t>microscope.</t> Scale bar = 20 μm.
    Eclipse Ti E C2 Confocal Laser Scanning Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ti+e+microscope/C2%2B/pmc12811640-78-16-23
    Average 99 stars, based on 1 article reviews
    eclipse ti e c2 confocal laser scanning microscope - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    99
    Nikon nikon ti e microscope
    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon <t>Eclipse</t> <t>Ti-E</t> <t>C2+</t> <t>microscope.</t> Scale bar = 20 μm.
    Nikon Ti E Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ti+e+microscope/Objectives/pmc12887731-34-8-8
    Average 99 stars, based on 1 article reviews
    nikon ti e microscope - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    99
    Nikon eclipse ti e c2 microscope
    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon <t>Eclipse</t> <t>Ti-E</t> <t>C2+</t> <t>microscope.</t> Scale bar = 20 μm.
    Eclipse Ti E C2 Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ti+e+microscope/C2%2B/pmc12811640-185-7-6
    Average 99 stars, based on 1 article reviews
    eclipse ti e c2 microscope - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    86
    Servicebio Inc transmission electron microscope ar ti cl e
    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon <t>Eclipse</t> <t>Ti-E</t> <t>C2+</t> <t>microscope.</t> Scale bar = 20 μm.
    Transmission Electron Microscope Ar Ti Cl E, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ti+e+microscope/electron+fixative+microscopy/pm42098334-90-1-26
    Average 86 stars, based on 1 article reviews
    transmission electron microscope ar ti cl e - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    99
    Nikon ti e eclipse inverted microscope
    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon <t>Eclipse</t> <t>Ti-E</t> <t>C2+</t> <t>microscope.</t> Scale bar = 20 μm.
    Ti E Eclipse Inverted Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ti+e+microscope/Objectives/pmc12984021-137-7-6
    Average 99 stars, based on 1 article reviews
    ti e eclipse inverted microscope - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    99
    Nikon nikon ti e inverted microscope
    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon <t>Eclipse</t> <t>Ti-E</t> <t>C2+</t> <t>microscope.</t> Scale bar = 20 μm.
    Nikon Ti E Inverted Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ti+e+microscope/Objectives/bio_rxiv__64898__2026__03__26__714479-265-13-13
    Average 99 stars, based on 1 article reviews
    nikon ti e inverted microscope - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    99
    Nikon nikon eclipse ti e microscope
    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon <t>Eclipse</t> <t>Ti-E</t> <t>C2+</t> <t>microscope.</t> Scale bar = 20 μm.
    Nikon Eclipse Ti E Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ti+e+microscope/Objectives/bio_rxiv__64898__2026__03__26__714479-267-6-6
    Average 99 stars, based on 1 article reviews
    nikon eclipse ti e microscope - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    99
    JEOL acceleration voltage ar ti cl e
    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon <t>Eclipse</t> <t>Ti-E</t> <t>C2+</t> <t>microscope.</t> Scale bar = 20 μm.
    Acceleration Voltage Ar Ti Cl E, supplied by JEOL, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ti+e+microscope/JEM-2100F+Transmission+Electron+Microscope/pm41896244-198-37-32
    Average 99 stars, based on 1 article reviews
    acceleration voltage ar ti cl e - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    99
    Nikon eclipse ti e inverted fluorescent microscope
    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon <t>Eclipse</t> <t>Ti-E</t> <t>C2+</t> <t>microscope.</t> Scale bar = 20 μm.
    Eclipse Ti E Inverted Fluorescent Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ti+e+microscope/Objectives/bio_rxiv__64898__2026__03__24__714019-126-21-20
    Average 99 stars, based on 1 article reviews
    eclipse ti e inverted fluorescent microscope - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope. Scale bar = 20 μm.

    Journal: Biofilm

    Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7

    doi: 10.1016/j.bioflm.2025.100335

    Figure Lengend Snippet: Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope. Scale bar = 20 μm.

    Article Snippet: After five days of static incubation at 30 °C, three-dimensional biofilm structures were observed using an Eclipse Ti-E C2+ confocal laser scanning microscope (Nikon) equipped with CFI Plan Apo VC 20x/1.2 and CFI Plan Apo VC 60x/1.2 WI objective lenses.

    Techniques: Staining, Mutagenesis, Fluorescence, Microscopy

    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope. Scale bar = 20 μm.

    Journal: Biofilm

    Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7

    doi: 10.1016/j.bioflm.2025.100335

    Figure Lengend Snippet: Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope. Scale bar = 20 μm.

    Article Snippet: The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope.

    Techniques: Staining, Mutagenesis, Fluorescence, Microscopy